Calpain 1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD; 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS; 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.

Description:
Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit (CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Synonyms:
Ca2 activated neutral protease antibody, Calcium activated neutral proteinase 1 antibody, Calcium activated neutral proteinase antibody, Calcium activated neutral proteinase small subunit antibody, Calcium dependent protease small subunit 1 antibody, Calcium dependent protease small subunit antibody, Calcium-activated neutral proteinase 1 antibody, Calpain 1(mu/I) large subunit antibody, Calpain 1 antibody, Calpain 1 catalytic subunit antibody, Calpain 1 large subunit antibody, Calpain Large Polypeptide L1 antibody, Calpain Large Polypeptide L1 antibody, Calpain mu type antibody, Calpain mu-type antibody, Calpain regulatory subunit antibody, Calpain small subunit 1 antibody, Calpain-1 catalytic subunit antibody, Calpain-1 large subunit antibody, Calpain1 antibody, CAN1_HUMAN antibody, CANP 1 antibody, CANP antibody, CANP small subunit antibody, CANPL 1 antibody, CANPL1 antibody, CAPN 1 antibody, CAPN1 antibody, Cell proliferation inducing protein 30 antibody, Cell proliferation-inducing gene 30 protein antibody, Micromolar Calpain antibody, Micromolar-calpain antibody, Mu Calpain antibody, muCANP antibody, muCL antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.