CaMKII α polyclonal, anti-human, mouse, rat

Background:
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.

Alternative Name:
CaM kinase II subunit alpha, CaMK-II subunit alpha, Alpha CaMKII, Calcium calmodulin dependent protein kinase II, Calcium/calmodulin dependent protein kinase II alpha B subunit, Calcium/calmodulin dependent protein kinase type II alpha chain, Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, Calcium/calmodulin-dependent protein kinase II alpha, Calcium/calmodulin-dependent protein kinase II-alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/calmodulin-dependent protein kinase type IIA, CaM kinase II alpha chain, CaM kinase II alpha subunit, CaM kinase II subunit alpha, CaMK II alpha subunit, CaMK-II subunit alpha, Camk2a, CAMKA, CaMKII, CaMKIINalpha, EC 2.7.11.17, KCC2A, KIAA0968, MGC123320, MGC139375, MGC155201, mKIAA0968, PK2CDD, PKCCD, R74975, zgc:112538, zgc:123320,

Application Dilution:
WB:1:1,000-1:2,000
FC:1:50-1:100
IP:1:10-1:50

Specificity: CaMKII α polyclonal antibody detects endogenous levels of CaMKII α protein.

Immunogen:
recombinant protein

MW: 54 kDa

Swiss-Prot: Q9UQM7(Human) P11798(Mouse) P11275(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.