CAMKIV polyclonal, anti-human, mouse, rat

Background:
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.

Alternative Name:
CaMK IV, CAMK4, CAMK, CAMK-GR, CAMKIV, Brain Ca(2+) calmodulin dependent protein kinase type 4, Brain Ca(2+) calmodulin dependent protein kinase type IV, Brain Ca++-calmodulin dependent protein kinase type IV, Calcium / calmodulin dependent protein kinase type 4 catalytic chain, Calcium / calmodulin dependent protein kinase type IV catalytic chain, Calcium/calmodulin dependent protein kinase IV, Calcium/calmodulin dependent protein kinase type IV, Calcium/calmodulin-dependent protein kinase type IV, CAM kinase 4, CAM kinase GR, CAM kinase IV, CAM kinase-GR, CaMK 4, CAMK GR, CaMK IV, Camk4, CaMKGR, IV, KCC4, MGC36771,

Application Dilution:
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: CAMKIV polyclonal antibody detects endogenous levels of CAMKIV protein.

Immunogen:
Recombinant protein corresponding to the C-terminus of human CAMKIV.

MW: 52 kDa

Swiss-Prot: Q16566(Human) P08414(Mouse) P13234(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.