Canine Alpha-Fetoprotein (AFP) Detection Kit

Use: Used to detect the concentration of canine alpha-fetoprotein (AFP) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-canine alpha-fetoprotein (AFP) antibody (solid phase antibody), add canine alpha-fetoprotein (AFP) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of canine alpha-fetoprotein (AFP) in the sample to be tested. Fitting the calibrator curve can calculate the concentration of canine alpha-fetoprotein (AFP) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.