Canine Cholecystokinin (CCK) Detection Kit

Use: Used to detect the concentration of canine cholecystokinin (CCK) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled canine cholecystokinin (CCK) is used, and the purified anti-canine cholecystokinin (CCK) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody inhibits the competitive reaction with biotin-labeled canine cholecystokinin (CCK) and non-labeled antigen (calibrator or specimen). The binding amount of antibody and biotin-labeled canine cholecystokinin (CCK) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled canine cholecystokinin (CCK), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated canine cholecystokinin (CCK) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated canine cholecystokinin (CCK)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of canine cholecystokinin (CCK) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of canine cholecystokinin (CCK) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.