Canine Hydroxyproline (HyP) Detection Kit

Use: Used to detect the concentration of canine hydroxyproline (HyP) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled canine hydroxyproline (HyP) is used, and the purified anti-canine hydroxyproline (HyP) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody inhibits the competitive reaction with biotin-labeled canine hydroxyproline (HyP) and non-labeled antigen (calibrator or specimen). The binding amount of antibody and biotin-labeled canine hydroxyproline (HyP) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled canine hydroxyproline (HyP), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated canine hydroxyproline (HyP) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated canine hydroxyproline (HyP)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of canine hydroxyproline (HyP) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of canine hydroxyproline (HyP) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.