Capping Protein alpha3 a3 N-terminal specific polyclonal, anti-mouse, human, bovine

Quantity: 25 µl

Background:
The F-actin capping protein is a heterodimer composed of two unrelated subunits: alpha and beta. Neither of the subunits shows sequence similarity to other filament-capping proteins. The alpha subunit is a protein of about 268 to 286 amino acid residues, their sequences are well conserved in eukaryotic species.

Synonyms:
CAPZA3, Gsg3, CAPPA3

Isotype: Guinea Pig Polyclonal (MVB4)

Immunogen:
Synthetic N-terminal domain (aa 2-17: SLSVLSRKEKEKVIHR-C) of mouse F-actin ?3 sub- unit, coupled to KLH (for sequence data see below ref. Tanaka et al., 1994)

Form: Stabilized Serum

Specificity:
Specific for F-actin capping protein (?3 subunit; MW 32,967, pI 6.91 calculated from sequence data; Mr 31,000 on SDS-PAGE; for different sequence data MW 35,024 and pI 7.61 see Tanaka et al.)

Reactivity: mouse, human, bovine

Applications: Suitable for immunoblotting (Western)

Working DIlution: 1:2,000 for Western blotting (ECL)

Storage: 2-8C

References:
- Von Buelow, M..et al. (1997) Exp. Cell Res 233: 216-224
- Tanaka, H. et al. (1994) FEBS Lett 355: 4-10