Cardif (clone Adri-1), anti-human

Decription:
RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.

Synonyms: CARD Adapter Inducing Interferon-beta, IPS-1, MAVS, Mitochondrial Antiviral Signaling Protein, VISA, Virus-induced Signaling Adapter, Interferon-beta Promoter Stimulator Protein 1

Clone: Adri-1

Isotype: Mouse IgG2b

Immunogen: Recombinant human Cardif (aa 160-450).

Reactivity: human

Specificity: Recognizes human Cardif.

Form: Purified, in PBS containing 10% glycerol and 0.02% sodium azide.

Applications:
Immunocytochemistry: (1:100) Immunohistochemistry: (paraffin sections) Immunoprecipitation: (1:200)
Western Blot: (1:1'000)

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)