Caspase-3 (P17) polyclonal, anti-rat, mouse

Quantity: 100 µg

Background:
CASP3(Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease(caspase) family. Tiso et al.(1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al.(1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al.(1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.

Description:
Rabbit IgG polyclonal antibody for Caspase-3 (CASP3) detection. Tested with WB, IHC-P in Mouse, Rat.

Synonyms:
A830040C14Rik antibody, Apopain antibody, Apopain precursor antibody, CASP 3 antibody, CASP-3 antibody, CASP3 antibody, Casp3a antibody, Caspase 3 antibody, Caspase 3 apoptosis related cysteine protease antibody, Caspase 3 p17 subunit antibody, Caspase 3, apoptosis-related cysteine peptidase antibody, Caspase 3, apoptosis-related cysteine protease antibody, Caspase 3, apoptosis-related cysteine protease a antibody, Caspase-3 subunit p17 antibody, Caspase3 antibody, CC3 antibody, CPP 32 antibody, CPP-32 antibody, CPP32 antibody, CPP32B antibody, Cysteine protease CPP32 antibody, EC 3.4.22.56 antibody, Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody, ICE3 antibody, LICE antibody, mldy antibody, OTTHUMP00000165052 antibody, OTTHUMP00000165053 antibody, OTTHUMP00000165054 antibody, PARP cleavage protease antibody, Procaspase3 antibody, Protein Yama antibody, SCA 1 antibody, SCA-1 antibody, SCA1 antibody, SREBP cleavage activity 1 antibody, Yama antibody, Yama protein antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17)(67-81aa TDVDAANLRETFMGL), different from the related rat sequence by one amino acid.A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17)(67-81aa TDVDAANLRETFMGL), different from the related rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: rat.
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.