Caspase 3 polyclonal, anti-human

Background:
Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

Alternative Name:
CASP-3, CPP-32, SCA-1, CASP3, Apopain, CASP 3, CASP-3, CASP3, CASP3, Caspase 3, Caspase-3 subunit p12, CPP 32, CPP-32, CPP32B, Cysteine protease CPP32, PARP cleavage protease, Protein Yama, SCA-1, SCA1, SREBP cleavage activity 1, Yama,

Application Dilution:
WB:1:1,000-1:2,000
IHC:1:50-1:200

Specificity: Caspase 3 polyclonal antibody detects endogenous levels of Caspase 3 protein.

Immunogen:
recombinant protein

MW: 35 kDa

Swiss-Prot: P42574(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.