Caspase 3 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Caspase 3(caspase 3, apoptosis-related cysteine peptidase) is a caspase protein that interacts with caspase 8 and caspase 9, also known as Caspase-3, PARP CLEAVAGE PROTEASE, APOPAIN, CPP32, CPP32B, YAMA. It is a member of the cysteine-aspartic acid protease(caspase) family. PCR analysis of 16 human tissues revealed expression of full-length CASP3, as well as CASP3s at somewhat lower levels, in all tissues tested. Western blot analysis of 3 cell lines revealed the prominent CASP3 band at 32 kD and CASP3s at 20 kD. Several human cancer cell lines showed coexpression of both variants at the mRNA and protein levels. Overexpression of the catalytically inactive CASP3s by human kidney cells offered some resistance to inducers of apoptosis, and CASP3s accumulation could be enhanced with addition of proteasome inhibitors. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Alternative splicing of this gene results in two transcript variants that encode the same protein. Encoded by the CASP3 gene, CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Nicholson et al. developed a potent peptide aldehyde inhibitor and showed that it prevented apoptotic events in vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death.

Description:
Rabbit IgG polyclonal antibody for Caspase-3 (CASP3) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
A830040C14Rik antibody, Apopain antibody, Apopain precursor antibody, CASP 3 antibody, CASP-3 antibody, CASP3 antibody, CASP3_HUMAN antibody, Casp3a antibody, Caspase 3 antibody, Caspase 3 apoptosis related cysteine protease antibody, Caspase 3 p12 subunit antibody, Caspase 3 p17 subunit antibody, Caspase 3, apoptosis-related cysteine peptidase antibody, Caspase 3, apoptosis-related cysteine protease antibody, Caspase 3, apoptosis-related cysteine protease a antibody, Caspase-3 subunit p12 antibody, Caspase3 antibody, CC3 antibody, CPP 32 antibody, CPP-32 antibody, CPP32 antibody, CPP32B antibody, Cysteine protease CPP32 antibody, EC 3.4.22.56 antibody, Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody, ICE3 antibody, LICE antibody, mldy antibody, OTTHUMP00000165052 antibody, OTTHUMP00000165053 antibody, OTTHUMP00000165054 antibody, PARP cleavage protease antibody, Procaspase3 antibody, Protein Yama antibody, SCA 1 antibody, SCA-1 antibody, SCA1 antibody, SREBP cleavage activity 1 antibody, Yama antibody, Yama protein antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminal of human Caspase 3(207-220aa RNSKDGSWFIQSLC), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.A synthetic peptide corresponding to a sequence at the C-terminal of human Caspase 3(207-220aa RNSKDGSWFIQSLC), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.