Caspase-8 polyclonal, anti-human

Background:
Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.

Alternative Name:
CASP-8, FLICE, MACH, CASP8, ALPS2B, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, Apoptotic cysteine protease, Apoptotic protease Mch-5, Apoptotic protease Mch5, CAP4, CASP-8, CASP8, CASP8, Caspase 8, Caspase 8 apoptosis related cysteine peptidase, Caspase-8 subunit p10, CED 3, FADD Like ICE, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, FLICE, FLJ17672, ICE-like apoptotic protease 5, MACH alpha 1/2/3 protein, MACH, MACH beta 1/2/3/4 protein, MCH5, MGC78473, MORT1 associated ced 3 homolog, MORT1-associated CED-3 homolog, OTTHUMP00000163717, OTTHUMP00000163720, OTTHUMP00000163724, OTTHUMP00000163725, OTTHUMP00000165062, OTTHUMP00000165063, OTTHUMP00000165064, OTTHUMP00000206552, OTTHUMP00000206582,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Caspase-8 polyclonal antibody detects endogenous levels of Caspase-8 protein.

Immunogen:
recombinant protein

MW: 55/46 kDa

Swiss-Prot: Q14790(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.