Caspase-8 polyclonal, anti-human, mouse, rat, hamster, dog, bovine

Protein Family: Peptidases

Pathway and Disease: Cell Growth and Death, Immune System, Neurodegenerative Disorders

Description:
Caspase-8 is the most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Caspase-8 binding to the adapter molecule FADD recruits the complex to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) triggers caspase-8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. The enzyme then cleaves and activates caspase-3, caspase-4, caspase-6, caspase-7, caspase-9 and caspase-10. Granzyme B and CPP32 cleave caspase-8 at Asp 315 and Asp 330 respectively to generate the two active subunits.

Alternate Names: Caspase-8, CASP-8, ICE-like apoptotic protease 5, MORT1-assiociated CED-3 homolog, MACH, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, FLICE, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP8, MCH5

Application Notes: E: 1:500-1:1,000; WB: 1:200-1:500; IHC: 1:200-1:500; IF: 1:100-1:500

Accession No.: Q14790

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of rodent Caspase-8.

Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 25% glycerol, 1 mg/ml BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.

Product Citations:
BC Soner et al. 2014. Int J Mol Med. 34(5): 1249-56. PMID 25216351.