Caspase 9 polyclonal, anti-human

Quantity: 100 µg

Background:
CASP9(CASPASE9), also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The CASP9 gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in caspase-3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Its genomic coordinates(GRCh37) is 1:15,818,768-15,851,284. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the CASP9 gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c and dATP, an event that leads to caspase-9 activation. CASP9 activity increases dramatically upon association with the apoptosome complex. And the majority of Casp9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.

Description:
Rabbit IgG polyclonal antibody for Caspase-9 (CASP9) detection. Tested with WB, IHC-P in Human.

Synonyms:
APAF 3 antibody, APAF-3 antibody, APAF3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease activating factor 3 antibody, Apoptotic protease Mch 6 antibody, Apoptotic protease Mch-6 antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody, CASP-9 antibody, CASP9 antibody, CASP9_HUMAN antibody, Caspase 9 apoptosis related cysteine peptidase antibody, Caspase 9 Dominant Negative antibody, Caspase 9c antibody, Caspase-9 antibody, Caspase-9 subunit p10 antibody, Caspase9 antibody, ICE LAP6 antibody, ICE like apoptotic protease 6 antibody, ICE-LAP6 antibody, ICE-like apoptotic protease 6 antibody, MCH6 antibody, RNCASP9 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human Caspase 9(183-198aa SNIDCEKLRRRFSSLH).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.