Cattle IGFBP4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Insulin Like Growth Factor Binding Protein 4(IGFBP4) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 4(IGFBP4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor Binding Protein 4(IGFBP4) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Apoptosis, Tumor immunity

Alternative Names:
BP4, HT29-IGFBP, IBP4

Sample Type:
Serum, plasma and other biological fluids..

Assay Type: Competitive Inhibition

Standard: 6000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only