Cattle SPATA4 (Spermatogenesis-associated protein 4) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spermatogenesis-associated protein 4 (SPATA4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Spermatogenesis-associated protein 4 (SPATA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spermatogenesis-associated protein 4 (SPATA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spermatogenesis-associated protein 4 (SPATA4) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Reproductive science,

Alternative Names:
SPATA4

Sample Type:
serum, plasma, tissue homogenates

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only