Cattle TGFb1 (Transforming Growth Factor Beta 1) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 1(TGFb1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1(TGFb1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1(TGFb1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Tumor immunity, Infection immunity

Alternative Names:
TGF Beta 1，TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide

Sample Type:
serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay Type: Competitive Inhibition

Standard: 1000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only