Cattle TLR2 (Toll-like Receptor 2) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll-like Receptor 2(TLR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll-like Receptor 2(TLR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll-like Receptor 2(TLR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll-like Receptor 2(TLR2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Tumor immunity, Infection immunity, Immune molecule, Pulmonology

Alternative Names:
CD282, TIL4, Toll/interleukin-1 receptor-like protein 4

Sample Type:
tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only