CD106 (VCAM-1) (clone 4B9), anti-human
€564.00
In stock
SKU
ARP-21-5262
Catalog Number: 21-5262
Size: 100 µg
Isotype: Mouse IgG1
Size: 100 µg
Isotype: Mouse IgG1
Background:
Vascular cell adhesion molecule-1 (VCAM-1) is induced on Human umbilical vein endothelium (HUVE) by recombinant Human tumor necrosis factor - alpha (rh TNF-alpha), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigRation at sites of inflammation and immune reaction.
Clone: 4B9
Isotype: Mouse IgG1
Source: 4B9 is a Mouse IgG1 monoclonal antibody geneRated by immunization of Balb/c mice with Human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant Human (rh) TNF-alpha (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.
Reactivity: human
Specificity: The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
Form: Each vial contains 100 ul of 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
Applications: Flow Cytometry, Immunohistochemistry (frozen), Immunoprecipitation
Storage: Store at 4C, or in small aliquots at -20C.
Vascular cell adhesion molecule-1 (VCAM-1) is induced on Human umbilical vein endothelium (HUVE) by recombinant Human tumor necrosis factor - alpha (rh TNF-alpha), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigRation at sites of inflammation and immune reaction.
Clone: 4B9
Isotype: Mouse IgG1
Source: 4B9 is a Mouse IgG1 monoclonal antibody geneRated by immunization of Balb/c mice with Human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant Human (rh) TNF-alpha (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.
Reactivity: human
Specificity: The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
Form: Each vial contains 100 ul of 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
Applications: Flow Cytometry, Immunohistochemistry (frozen), Immunoprecipitation
Storage: Store at 4C, or in small aliquots at -20C.
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