CD11a (activitor) (clone NKI(SPV)-L16), anti-human

Background:
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a Human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common beta-chains (CD18). LFA-1 also has a distinct alpha-chain (CD11a). LFA-1 is expressed on lymphocytes, monocytes and granulocytes; it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstRated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to prolifeRate. A unique antibody NKI(SPV)-L16, reacting with the a-chain of the Human leukocyte function-associated Ag- 1 (LFA- 1) has been made available. This antibody stimulates homotypic cell-cell interactions in a manner very similar to 12-0-tetradecanoyl-phorbol1-3 -acetate (TPA), in contrast to other anti-LFA-1 mAb which inhibit cell aggregation. Immunoprecipitation and enzyme digestion studies revealed that NKI-L16 recognizes a unique epitope on the cy-chain of LFA-1,most likely situated close to the transmembrane segment of the molecule. It is hypothesized that NKI-L16 or TPA can cause the LFA-1 molecule to convert from an inactive to an active configuRation, thereby permitting binding of LFA-1 1 to its natural ligand. This mAb directed, in contrast to other anti-LFA-1 antibodies, stimulates Rather than inhibits homotypic cell aggregate formation, but does not affect cell proliferation.

Clone: NKI(SPV)-L16

Isotype: Mouse IgG2a

Source: This antibody was raised by immunization of Balb/c mice with monocytes. This unique activation dependent epitope of the SPV-L16 antibody on the a-chain of LFA-1 is relatively insensitive to trypsin treatment and thus most likely loCated close to the transmembrane segment of the protein.

Reactivity: human

Specificity: This antibody recognizes an activation epitope of CD11a and has been essential to define inside-out signaling of intergrin. Homotypic cell aggregate formation is stimulated (see also Background).

Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.

Applications: Flow Cytometry, Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.