CD11b (clone HI11b), anti-human

Protein Family: CAM Ligands, Cell Adhesion Molecules, Cellular Antigens

Pathway and Disease: Cell Motility, Immune System, Signaling Molecules and Interaction

Description:
The CD11b (HI11b) antibody recognizes the 165-kDa integrin αM-chain associated with CD18, the 95-kDa integrinβ2, which form a heterodimer glycoprotein called CD11b/CD18 (Mac-1). Leukocytes express four CD11/CD18 integrins which include a common β2 subunit and distinctive α chains. Besides CD11b/CD18, the other three integrins are known as CD11a/CD18, CD11c/CD18 and CD11d/CD18. Expression of the CD11b subunit on the leukocyte cell surface requires the presence of the CD18 subunit. Patients who have mutated CD18 genes do not express cell membrane Mac-1 (or the other β2 integrins), a condition known as leukocyte adhesion deficiency 1 (LAD-1), in which patients have major problems with bacterial infections. Mac-1 is highly expressed on neutrophils and monocytes/macrophages, a subset of NK cells, and activated lymphocytes. Mac-1 operates as a phagocytic receptor, has a role in myeloid cell transmigration and is also a receptor for iC3b, CD54 (ICAM-1), CD102 (ICAM-2) and fibrinogen.

Alternate Names: Integrin alpha-M, Leukocyte adhesion receptor MO1, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, Neutrophil adherence receptor, CD11 antigen-like family member B, CD11b antigen, ITGAM, CD11B, CR3A

Application Notes: Purified antibody is suitable for immunohistochemistry with acetone-fixed frozen sections and formalin-fixed paraffin tissues. E: 1:500-1:1,000; FC: 1:200-1:1,000; IHC: 1:200-1:500

Accession No.: P11215

Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.

Product Citations:
[1] Zalfa C et al. 2016. Cell Death Dis. 7(8): e2311. PMID 27512952.