CD11b (clone NR-12C) FITC, anti-mouse

Background:
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Decription:
Rat monoclonal Antibody to Mouse CD11b, FITC conjugate

Synonyms: ITGAM, CD11b, integrin, alpha M (complement component 3 receptor 3 subunit)

Clone: NR-12C

Isotype: Rat IgG

Immunogen: Thioglycollate-induced peritoneal cells

Reactivity: mouse

Specificity: Mouse CD11b

Form: Affinity Purified, in PBS containing 0.5% BSA and 0.1% Sodium Azide

Applications: Staining antibody

Working Dilution:
For flow cytometry, add 5-10 ul per 106 cells.
Note: Different sources of tissue macrophages may express different levels of CD11b.

Storage: 2-8C for immediate use; or at -20C (aliquot) Avoid freeze-thaw cycles