CD147/Emmprin/Basigin (Extracellular region) (clone M036), anti-human

Background:
CD147 (Basigin/EMMPRIN) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. CD147 is a glycosylated protein with four known isoforms with isoform 2 being the most abundantly expressed. In many cancers, CD147 is overexpressed and stimulates the secretion of extracellular matrix metalloproteinases by tumor fibroblasts, which can promote tumor progression. CD147 is co-expressed with the amino acid transporter, CD98h, in metabolically active cells such as lymphocytes, macrophages, and tumor cells. CD147 interaction with caveolin and cyclophilins regulates its functions, and this protein may function outside of tumor cells through cell shedding and microvesicle release.

Sequence: Clone (M036) was generated from a proprietary antigen related to native human CD147 expressed in NCI-H1915 lung cancer cell line.
*For more information, see UniProt Accession P35613

Specificity: Clone M036 mouse monoclonal antibody detects a 38-58 kDa* protein on SDS-PAGE immunoblots of human BEAS, NCIH28, NCI-H1299, LNCaP, and A431 cells, as well as human brain tissues. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

Application dilution:
WB 1:2000
ELISA (Cap) 1:100
ICC 1:100
IP 1:100
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.