CD173 (H type 2 Antigen) (clone BRIC 231) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human H type 2 Antigen Affinity Purified. H antigens are carried on the non-reducing termini of the car­bohydrates of glycoproteins and glycolipids. The H determinant structure is Fuc(a1-2) Gal(beta1)-R. Type 1 and type 2 H (CD 173) are deter­mined by the subterminal (peripheral core) carbohydrate sequence. In H type 1 it is Fuc (a1-2) Gal(beta1-3) GlcNAc, in H type 2 it is Fuc(a1-2) Gal(beta1-4) GlcNAc. H is the precursor of the A and B histo-blood group antigens, which are formed by the addition of GalNAc(a1-3) or Gal(a1-3) respectively, to the galactose of H. In man, H active substances are found on the erythrocytes, cells and tissues, and in the body fluids, linked to lipids (glycos­phingolipids) or to proteins (glycoproteins). In various animals, H antigens occur in the cells and tissues, but not generally on erythrocytes. The synthesis of H type 1 and H type 2 in man in different tissues is controlled by either of the two linked genes Se and H, which code for 2-fucosyl transferases.

Synonyms: H type 2 Antigen, CD173

Clone: BRIC 231

Isotype: Mouse IgG1 kappa light chain

Immunogen: H (ISBT No. 18001) / CD 173. BRIC 231 was made in response to immunisation with HEL cells.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Immunofluorescence, Immunoblotting, Immunocytochemical techniques.

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Clausen, H., Hakomori, S., Vox Sang. 56, 1-20 (1989)
2. Oriol, R., Le Pendu, J., Mollicone, R., Vox Sang. 51, 161-171 (1986