CD233 (Band 3) cytoplasmic domain (clone BRIC 155) Culture supernatant, anti-human
€204.00
In stock
SKU
ARP-08-9449-1
Catalog Number: 08-9449-1
Size: 0.5 ml
Isotype: Mouse IgG2b, kappa light chain
Size: 0.5 ml
Isotype: Mouse IgG2b, kappa light chain
Decription:
Mouse monoclonal antibody to human CD233/(Band3) extracellular domain Culture supernatant. CD233 (also known as erythrocyte band 3, EPB3, anion exchange protein 1, AE1, solute carrier family 4, SLC4A1) is an integral membrane protein in human erythrocytes, present at approximately 106 copies per human erythrocyte. It comprises two domains that are structurally and functionally distinct. The cytoplasmic N-terminal 40kDa domain has binding sites for erythrocyte cytoskeletal proteins, namely ankyrin and protein 4.2, which help to maintain the mechanical properties and integrity of the erythrocyte. This domain also binds a number of other erythrocyte peripheral proteins. The 55kDa glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments which function as a chloride/bicarbonate anion exchanger involved in carbon dioxide transport. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. CD233 associates with the erythrocyte membrane protein glycophorin A (GPA) which promotes the correct folding and translocation during biosynthesis of CD23. Many CD233 mutations are known in man and these mutations can lead to two types of disease; destabilization of the erythrocyte membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other CD233 mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. The CD233 gene is located on chromosome 17q21-q2.
Synonyms: CD233, erythrocyte band 3, EPB3, anion exchange protein 1, AE1, solute carrier family 4, SLC4A1
Clone: BRIC 155
Isotype: Mouse IgG2b, kappa light chain
Immunogen: BRIC 155 was made in response to endo F-treated 55 kD tryptic bound domain of band 32. The sequences reactive with BRIC 155 were degraded by carboxypeptidase Y treatment of intact protein or fragments of protein containing the C-terminus.
Reactivity: human
Form: Culture Supernatant
Applications: Immunoblotting, Flow Cytometry, Immunofluorescence
Working Dilution: Optimal dilution should be performed by user
Storage: 2-8C for immediate use, or at -20C (aliquot)
References:
1. Bruce L.J. and Tanner M.J.A. (Review) (2001) PROW 2, 9-17.
2. Wainwright S.D., Tanner M.J.A., Martin G.E.M., Yendle J.E. and Holmes C. (1989) Biochem. J.258, 211-220.
3. Wainwright S.D., Mawby W.J. and Tanner M.J.A. (1990) Biochem. J. 272, 265-268.
4. Bruce L. (2006) Blood cells, Molecules and disease 36, 331-336.
5. Toye A.M. et al (2004) J. Cell Science 117, 1399-1410.
6. Beckmann R. et al (1998) Blood 92, 4428-4438.
7. Beckmann R. et al (2001) Blood 97, 2496-2505.
8. Bruce L. J. et al (2003) Blood 101, 4180-4188
Mouse monoclonal antibody to human CD233/(Band3) extracellular domain Culture supernatant. CD233 (also known as erythrocyte band 3, EPB3, anion exchange protein 1, AE1, solute carrier family 4, SLC4A1) is an integral membrane protein in human erythrocytes, present at approximately 106 copies per human erythrocyte. It comprises two domains that are structurally and functionally distinct. The cytoplasmic N-terminal 40kDa domain has binding sites for erythrocyte cytoskeletal proteins, namely ankyrin and protein 4.2, which help to maintain the mechanical properties and integrity of the erythrocyte. This domain also binds a number of other erythrocyte peripheral proteins. The 55kDa glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments which function as a chloride/bicarbonate anion exchanger involved in carbon dioxide transport. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. CD233 associates with the erythrocyte membrane protein glycophorin A (GPA) which promotes the correct folding and translocation during biosynthesis of CD23. Many CD233 mutations are known in man and these mutations can lead to two types of disease; destabilization of the erythrocyte membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other CD233 mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. The CD233 gene is located on chromosome 17q21-q2.
Synonyms: CD233, erythrocyte band 3, EPB3, anion exchange protein 1, AE1, solute carrier family 4, SLC4A1
Clone: BRIC 155
Isotype: Mouse IgG2b, kappa light chain
Immunogen: BRIC 155 was made in response to endo F-treated 55 kD tryptic bound domain of band 32. The sequences reactive with BRIC 155 were degraded by carboxypeptidase Y treatment of intact protein or fragments of protein containing the C-terminus.
Reactivity: human
Form: Culture Supernatant
Applications: Immunoblotting, Flow Cytometry, Immunofluorescence
Working Dilution: Optimal dilution should be performed by user
Storage: 2-8C for immediate use, or at -20C (aliquot)
References:
1. Bruce L.J. and Tanner M.J.A. (Review) (2001) PROW 2, 9-17.
2. Wainwright S.D., Tanner M.J.A., Martin G.E.M., Yendle J.E. and Holmes C. (1989) Biochem. J.258, 211-220.
3. Wainwright S.D., Mawby W.J. and Tanner M.J.A. (1990) Biochem. J. 272, 265-268.
4. Bruce L. (2006) Blood cells, Molecules and disease 36, 331-336.
5. Toye A.M. et al (2004) J. Cell Science 117, 1399-1410.
6. Beckmann R. et al (1998) Blood 92, 4428-4438.
7. Beckmann R. et al (2001) Blood 97, 2496-2505.
8. Bruce L. J. et al (2003) Blood 101, 4180-4188
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