CD233 (Band 3) polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.

Description:
Rabbit IgG polyclonal antibody for Band 3 anion transport protein (SLC4A1) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.

Synonyms:
AE 1 antibody, AE1 antibody, Anion exchange protein 1 antibody, Anion exchanger 1 antibody, B3AT_HUMAN antibody, Band 3 anion transport protein antibody, BND3 antibody, CD233 antibody, DI antibody, Diego blood group antibody, EMPB3 antibody, EPB3 antibody, Erythrocyte membrane protein band 3 antibody, Erythroid anion exchange protein antibody, FR antibody, Froese blood group antibody, RTA1A antibody, SLC4A1 antibody, Solute carrier family 4 anion exchanger member 1 antibody, Solute carrier family 4 member 1 antibody, SW antibody, Swann blood group antibody, Waldner blood group antibody, WD antibody, WD1 antibody, WR antibody, Wright blood group antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E. coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat Band 3, respectively.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.