CD235a (M antigen) (clone 6A7) FITC, anti-human

Decription:
Mouse monoclonal antibody to human M Antigen FITC conjugate. The blood group M antigen is a polymorphic antigen expressed on the major erythrocyte sialoglycoprotein, Glycophorin A (GPA, CD 235a). The complete amino acid sequence and sites of glycosylation of GPA are known. The M antigen is defined by serine and glycine at positions 1 and 5 respectively of GPA. Glycophorin A is heavily glycosylated with numerous O- glycans containing sialic acid. It is found on erythroid cells and the HEL erythroleukaemia cell line. There are approximately 2-10 x 105 GPA molecules per erythrocyte. Rare individuals lacking GPA are known.

Synonyms: M antigen, GPA, Glycophorin A, CD235a

Clone: 6A7

Isotype: Mouse IgG2a kappa light chain

Immunogen: Found on erythroid cells and the HEL erythroleukemia cell line.

Reactivity: human

Form: FITC-conjugate, in PBS buffer, pH 7.2 containing 0.1% sodium azide, 1% BSA

Applications: Flow Cytometry

Working Dilution: 2ul per test (may change from lot to lot)

Storage: 2-8C, do not freeze

References:
1. Reid ME, Lomas-Francis C. (1997) The blood group antigen facts book. Academic Press, London
2. Anstee, D.J., (1990) Vox Sang; 58: 1 20 (Review).
3. Bigbee W.L. et al., (1983) Mol. Immunol., 20: 1353-1362
4. Langlois R.G. et al.,(1990) Cytometry 11: 513-521