CD235b (N antigen) (clone BRIC 157) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human N antigen-Affinity purified. The blood group N antigen is expressed on the erythrocyte sialoglycoproteins Glycophorin A (GPA, where it is polymorphic, CD 235a) and Glycophorin B (GPB, CD 235b). The complete amino acid sequence of both of these highly homologous membrane glycoproteins is known. The nature of the amino acids at positions 1 and 5 (leucine and glutamic acid respectively) defines the N antigen on both GPA and GPB. Glycophorins A and B are heavily glycosylated with numerous O- glycans containing sialic acid. The reactivity of some anti-N reagents is affected by the degree of sialylation of the antigen. The antigen is found on erythroid cells and K562 and HEL erythroleukaemia cell lines. All normal erythrocytes express N antigen on GPB: MN and NN erythrocytes also express N antigen on GPA. There are approximately 2-10 x 105 GPA and 8-25 x 104 GPB molecules per erythrocyte. Rare individuals lacking GPA and GPB are known.

Synonyms: GPA, Glycophorin A, CD235a, Glycophorin B, GPB, CD235b, NN, MN

Clone: BRIC 157

Isotype: Mouse IgG1 kappa light chain

Immunogen: Found on erythroid cells, K562 and the HEL erythroleukemia cell lines. All normal erythrocytes express N antigen on GPB: MN and NN erythrocytes also express N antigen on GPA.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: ELISA, Immunoblotting

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Reid ME, Lomas-Francis C. (1997) The blood group antigen facts book. Academic Press, London
2. Anstee, D.J., (1990) Vox Sang; 58: 1 20 (Review).
3. Langlois R.G. et al.,(1990) Cytometry 11: 513-521