CD238 (Blood Group Kpbc Antigen) (clone BRIC 203) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human Blood Group Kpbc/CD238 Affinity purified. The Kpa, Kpb, and Kpc antigens are part of the Kell (CD 238) blood group system. The antigens of the Kell system are carried on an erythrocyte membrane glycoprotein of 93 kDa, which is firmly bound to the cytoskeleton. The antigen is found on human erythrocytes, liver sinusoidal cells4 and testis with weaker expression in a large number of other tissues such as brain and lymphoid tissues. Immunohistochemistry reveals human Kell protein is localized to the Sertoli cells of the testis and the follicular dendritic cells of the spleen and tonsil. On erythrocytes, Kell is linked by a single disulfide bond to XK. The absence of XK, as occurs in the McLeod phenotype, is associated with a set of clinical symptoms that include nerve and muscle disorders and red cell acanthocytosis. The Molecular Weight of Kell is120 kDa.

Synonyms: CD238, Kpa antigen, Kpb antigen, Kpc antigen

Clone: BRIC 203

Isotype: Mouse IgG1 kappa light chain

Immunogen: Recombinant Kell Antigen

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Flow Cytometry, Immunohistochemistry, Immunoblotting, Immunoprecipitation.

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
Marsh WL & Redman CM (1990) Transfusion 30, 158-167 (review)
Redman CM et al. (1984) Transfusion 24, 176-178
Lee S et al. (1991) Proc. Natl. Acad. Sci. USA 88, 6353-6357
Anstee DJ et al (1992) In protein blood group antigens of the human red cell, chap. 8 170-18
1. ed. Agre P & Cartron JP. Johns Hopkins University Press.
Parsons SF, et al. (1991) Transf. Med. 1 supp. 248 (abstract)
Parsons SF et al. (1993) Transf. Med., 3: 137-142