CD240D (Rh D Blood Group Antigen, ISBT4001) (clone BRAD-3) FITC, anti-human

Decription:
Human heterohybridoma to human Rh D (ISBT4001)/CD240. CD blood group-related antigen FITC conjugate. The Rh D antigen (Rh1 or Rho ) is clinically the most important of the Rh blood group system. It is expressed on the extracellular loops of a transmembrane polypeptide of around Mr 30000. Estimated numbers of Rh D sites recognised by BRAD-3 on Rh D positive cells are between 10000 to 14000 on CDe/cde (R1r) cells and 27000 to 33000 on cDE/cDE (R2R2) cells.

Synonyms: Rh D blood group antigen, CD240

Clone: BRAD-3

Isotype: Human IgG3 kappa light chain

Immunogen: Rh D antigen produced by an EBV transformed B cell line derived from the peripheral blood of immunized RhD negative donor.

Reactivity: human

Form: FITC-conjugate, in PBS buffer, pH 7.2 containing 0.1% sodium azide, 1% BSA

Applications: Flow Cytometry, Indirect Immunofluorescence

Working Dilution: 2ul per test (may change from lot to lot)

Storage: 2-8C, do not freeze

References:
Cartron, J-P, (1994) Blood Reviews 8, 199
Jones et al., (1996), Vox Sanguinis 71, 176-183
Kumpel et al., (1989) Br. J. Haematol. 71, 125
Jones J et al. (1995) Transfusion Medicine 5, 171-184
Lloyd-Evans et al., (1995), Transfusion Medicine 5, 171
Lloyd-Evans et al., (1996). Transfusion 36, 432-437
Lloyd-Evans P. et al, (1999) Transfusion Medicine 9 155-160
Kumpel B. (2001) Transfusion 41, 1059-1063
Chapman JF (1999) Working party of the BCSH Blood Transfusion and General Haematology Task Forces. Transfusion Medicine 9, 87-92
Austin E. et al. (2009) Guidelines for the estimation of fetomaternal haemorrhage. Working party of the BCSH Transfusion Taskforce. BCSH FMH Guidelines 2009 p1-23
Lloyd-Evans P. et al, (1999) Transfusion Medicine 9 suppl 1:33. 9.