CD244/2B4/SLAMF4 (Extracellular region) (clone M053), anti-human

Background:
CD244 (Natural killer (NK) cell receptor 2B4/SLAMF4) is an Ig superfamily signaling lymphocyte activation molecule (SLAM) receptor. Like all SLAM family receptors, it has an extracellular segment with two immunoglobulin (Ig)-like domains, and a cytoplasmic domain containing four immunoreceptor tyrosine-based switch motifs. CD244 does not act as a selfligand similar to other SLAM family receptors. It binds CD48, a transmembrane receptor ubiquitously expressed on hematopoietic cells. CD244 activity is controlled by the presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Downstream signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Activation of CD244 stimulates NK cell cytotoxicity, production of IFN-γ and granule exocytosis. CD244 is involved in the regulation of CD8+ T-cell proliferation, and inhibits inflammatory responses in dendritic cells (DCs). In cancers, CD244 shows increased expression in intratumoral DCs and myeloid suppressor cells, and anti-CD244 therapies may increase infiltrating T-cells and impair tumor growth.

Sequence: Clone (M053) was generated from a recombinant protein that included the extracellular region of human CD244 protein.
*For more information, see UniProt Accession Q9BZW8

Specificity: Clone M053 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human THP1 monocytes. The antibody prefers the native CD244 protein, but also detects denatured CD244. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

Application dilution:
ELISA (Cap) 1:100
ICC 1:200
IP 1:100
WB 1:500
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.