CD246 (ALK) polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
ALK(Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246(cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.

Description:
Rabbit IgG polyclonal antibody for ALK tyrosine kinase receptor (ALK) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
Alk antibody, ALK tyrosine kinase receptor antibody, ALK tyrosine kinase receptor precursor antibody, ALK/EML4 fusion gene, included antibody, ALK/NPM1 fusion gene, included antibody, ALK_HUMAN antibody, anaplastic lymphoma kinase(Ki-1) antibody, Anaplastic lymphoma kinase antibody, Anaplastic lymphoma kinase Ki 1 antibody, Anaplastic lymphoma kinase Ki1 antibody, Anaplastic lymphoma kinase p80 antibody, anaplastic lymphoma receptor tyrosine kinase antibody, CD246 antibody, CD246 antibody, CD246 antigen antibody, EC 2.7.10.1 antibody, Ki 1 antibody, Ki1 antibody, mutant anaplastic lymphoma kinase antibody, NBLST 3 antibody, NBLST3 antibody, Tcrz antibody, TFG/ALK antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human ALK(1571-1589aa LFRLRHFPCGNVNYGYQQQ), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the C-terminus of human ALK(1571-1589aa LFRLRHFPCGNVNYGYQQQ), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat.
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.