CD25 (IL-2 Receptor, IL-2R) (clone NR-72), anti-human

Background:
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency

Decription:
Mouse monoclonal Antibody to human IL-2 Receptor

Synonyms: CD25, IL2R

Clone: NR-72

Isotype: Mouse IgG2a

Immunogen: Con A-activated human T cells

Reactivity: human

Specificity: Human IL-2 Receptor

Form: Affinity Purified, in PBS containing 0.1% Sodium Azide

Applications: FACS analysis, Sorting

Working Dilution: 1:50 dilution will block 70-80% of CTLL proliferative response to 50 units of r.human IL-2 and will stain 70% of PHA-activated human T cells (by FACS) 10µl will stain 106 IL-2R positive cells for FACS analysis or sorting

Storage: 2-8C for immediate use; or at -20C (aliquot) Avoid freeze-thaw cycles