CD274 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
CD274(CD274 molecule), also known as PDCD1L1, PDL1, B7H1, CD274 molecule, cluster of differentiation(CD274) or B7 homolog 1(B7-H1), Programmed cell death 1 ligand 1, is a protein that in humans is encoded by the CD274 gene. Levels of B7H1 correlated with PTEN loss in glioblastoma specimens, and tumor-specific T cells lysed human glioma targets expressing wildtype PTEN more effectively than those expressing mutant PTEN. Sequence analysis predicted that it has 290-amino acid. immunoresistance in glioma is related to loss of the tumor suppressor PTEN and is mediated in part by B7H1.Programmed death ligand 1(PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Using microarray analysis and flow cytometry, Barber et al.(2006) found that Pd1 was highly upregulated by functionally exhausted CD8 T cells from mice infected with a lymphocytic choriomeningitis virus(LCMV) strain causing chronic infection, but not by functional memory CD8 T cells from mice infected with an LCMV strain causing acute infection. 

Description:
Rabbit IgG polyclonal antibody for Programmed cell death 1 ligand 1 (CD274) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
B7 H antibody, B7 H1 antibody, B7 homolog 1 antibody, B7-H1 antibody, B7H antibody, B7H1 antibody, CD 274 antibody, Cd274 antibody, CD274 antigen antibody, CD274 molecule antibody, MGC142294 antibody, MGC142296 antibody, OTTHUMP00000021029 antibody, PD L1 antibody, PD-L1 antibody, PD1L1_HUMAN antibody, PDCD1 ligand 1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL 1 antibody, PDL1 antibody, Programmed cell death 1 ligand 1 antibody, Programmed death ligand 1 antibody, RGD1566211 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human CD274(100-114aa QITDVKLQDAGVYRC), different from the related rat and mouse sequences by one amino acid.A synthetic peptide corresponding to a sequence at the N-terminus of human CD274(100-114aa QITDVKLQDAGVYRC), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.