CD28 (Phospho-Tyr218) polyclonal, anti-human, mouse

Specificity:
CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.

Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human CD28 around the phosphorylation site of Tyr218.

Immunogen Sequence:
Phospho

Modification Sites: H:Y218, M:Y216

MW (kDa): 25 kDa

NCBI Gene Symbol: CD28

Database Links:
Gene ID: 940
Swiss Prot No.: P10747
OMIM No.: 186760
Unigene No.: Hs.591629

Application Dilutions: WB: 1:500~1:1000, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.