CD295 (Leptin Receptor) polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
LEPR(Leptin receptor) also known as LEP-R, OBR, is a protein that in humans is encoded by the LEPR gene. The leptin hormone regulates adipose-tissue mass through hypothalamus effects on fullness and energy use. It acts through the leptin receptor(LEP-R), a single-transmembrane-domain receptor of the cytokine receptor family. By genetic mapping, Tartaglia et al.(1995) demonstrated that the Obr gene is located on mouse chromosome 4, in a 5.1-cM interval that contains the well-characterized recessive obesity mutation, diabetes(db). Chung et al.(1996) developed a genetic map of 1p in the region of the OBR gene. They mapped the OBR gene physically by radiation hybrid mapping and placed it on a contig composed of 10 adjacent YACs and 5 P1 artificial chromosomes(PACs). The location of the human homolog of Obr was predicted to be on 1p based on conserved linkage of most of the telomeric half of mouse chromosome 4 with human 1p. LEP-R functions as a receptor for the fat cell-specific hormone leptin. LEP-R has also been designated as CD295.

Description:
Rabbit IgG polyclonal antibody for Leptin receptor (LEPR) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
CD295 antibody, CD295 antibody, CD295 antigen antibody, Db antibody, Fa antibody, HuB219 antibody, LEP R antibody, LEP-R antibody, LEPR antibody, LEPR_HUMAN antibody, LEPRD antibody, LEPROT antibody, Leptin receptor antibody, Leptin receptor fatty antibody, Leptin receptor gene related protein antibody, Leptin receptor precursor antibody, OB R gene related protein antibody, OB receptor antibody, OB-R antibody, OB-RGRP antibody, obl antibody, Obr antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human Leptin Receptor(798-817aa HDHFIPIEKYQFSLYPIFME), different from the related mouse and rat sequences by two amino acids.A synthetic peptide corresponding to a sequence in the middle region of human Leptin Receptor(798-817aa HDHFIPIEKYQFSLYPIFME), different from the related mouse and rat sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.