CD3 FITC/CD16+56 PE/CD45 PerCP (clone 33-2A3, 3G8+B-A19, D3/9), anti-human RUO

Introduction:
The CD3 monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets.
This CD16 PE, clone 3G8 monoclonal antibody reacts with human CD16, which is also known as the low-affinity FcγRIII. CD16 exists as two distinct isoforms, FcγRIIIA and FcγRIIIB. In humans, FcγRIIIA is expressed as a polypeptide- anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor.
FcγRIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates.
CD56, expression of neural cell adhesion molecules (N-CAM) provides neurons with a means of attaching to and interacting with other cells and the extracellular matrix.
The CD45 monoclonal antibody is directed against the CD45-antigen, defined T200 or Leucocyte Common Antigen. The antibody reacts with all cells of the haemopoietic lineage, not with cells of other lineages.

Recomended usage:
Immunostep’s CD3/CD16+CD56/CD45, clones 33-2A3, 3G8/B-A19 and D3/9 is a monoclonal antibody intended for identification of Natural Killer (CD3-CD16+CD56+CD45+) and a subpopulation of T lymphocytes (CD3+). This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 10^6 cells.

Application: Flow Cytometry

Storage Temperature: Store in the dark at 2-8 °C

Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)