CD3 FITC/CD16+56 PE (clone 33-2A3, 3G8+B-A19), anti-human RUO

Introduction:
The CD3 monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.
This CD16 PE, clone 3G8 monoclonal antibody reacts with human and non-human primate CD16, which is also known as the low-affinity FcγRIII. CD16 exists as two distinct isoforms, FcγRIIIA and FcγRIIIB. In humans, FcγRIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. FcγRIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates. However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys. Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation

Recomended usage:
Immunostep’s CD3/CD16+CD56, clone 33-2A3, 3G8/B-A19 is a monoclonal antibody intended for identification of Natural Killer (CD3-CD16+CD56+) and a subpopulation of T lymphocytes (CD3+). This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 106 cells.

Application: Flow Cytometry

Storage Temperature: Store in the dark at 2-8 °C

Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)