CD3 FITC/CD8 PE/CD45 PerCP (clone 33-2A3, 143-44, D3/9), anti-human RUO

Introduction:
The CD3 monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.
The CD8 monoclonal antibody is directed against the CD8-antigen (T8-antigen),which is expressed on human T lymphocytes. The monoclonal antibody reacts with 20-30% of human peripheral T lymphocytes. The monoclonal antibody reacts with T lymphocytes with suppressor-cell activity in pokeweed mitogen- stimulated immunoglobulin production, as was shown in separation experiments (i.e. "panning").
The CD45 monoclonal antibody is directed against the CD45- antigen, defined T200 or Leucocyte Common Antigen. The antibody reacts with all cells of the haemopoietic lineage, not with cells of other lineages.

Recomended usage:
Immunostep’s CD3/CD8/CD45, is a monoclonal antibody intended for simultaneous detection and enumeration of lymphocytes. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 106 cells.

Application: Flow Cytometry

Storage Temperature: Store in the dark at 2-8 °C

Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)