CD304 (Neuropilin 1) polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor(VEGF) and semaphorin(see SEMA3A) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10.They localized the NRP1 gene to 10p12 using radiation hybrid mapping. Sulfation of the glucosamine-O-6 and iduronic acid-O-2 groups of heparin potentiated PGF2 binding to NRP1. NRP1 also bound PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

Description:
Rabbit IgG polyclonal antibody for Neuropilin-1 (NRP1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
A5 protein antibody, BDCA4 antibody, BLOOD DENDRITIC CELL ANTIGEN 4 antibody, CD 304 antibody, CD304 antibody, DKFZp686A03134 antibody, DKFZp781F1414 antibody, NEL like protein 1 antibody, Nel related protein 1 antibody, Neuropilin-1 antibody, Neuropilin1 antibody, Neuropilin1 transmembrane receptor antibody, NP1 antibody, NPN1 antibody, NRP 1 antibody, NRP antibody, NRP1 antibody, NRP1_HUMAN antibody, OTTHUMP00000020818 antibody, OTTHUMP00000020820 antibody, OTTHUMP00000020821 antibody, Vascular endothelial cell growth factor 165 receptor antibody, VEGF165R antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human Neuropilin 1(211-230aa LEIWDGFPDVGPHIGRYCGQ), different from the related rat and mouse sequences by one amino acid.A synthetic peptide corresponding to a sequence in the middle region of human Neuropilin 1(211-230aa LEIWDGFPDVGPHIGRYCGQ), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.