CD324 (E-Cadherin, Intercellular Junction Marker) (clone CDH1/1121), anti-human
€0.00
In stock
SKU
ARP-24-999-MSM1
Catalog Number: 24-999-MSM1
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Decription:
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as Ī �-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
Synonyms: Arc 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD324, CDH1, CDHE, E-Cad/CTF3, E-cadherin, ECAD, Epithelial cadherin, epithelial calcium dependent adhesion protein, Liver cell adhesion molecule (LCAM), Uvomorulin (UVO)
Clone: CDH1/1121
Isotype: Mouse IgG1, kappa
Immunogen: Recombinant fragment (119 Amino acid residues around aa 400-500) of human E-Cadherin protein
Reactivity: human. Others-not known
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as Ī �-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
Synonyms: Arc 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD324, CDH1, CDHE, E-Cad/CTF3, E-cadherin, ECAD, Epithelial cadherin, epithelial calcium dependent adhesion protein, Liver cell adhesion molecule (LCAM), Uvomorulin (UVO)
Clone: CDH1/1121
Isotype: Mouse IgG1, kappa
Immunogen: Recombinant fragment (119 Amino acid residues around aa 400-500) of human E-Cadherin protein
Reactivity: human. Others-not known
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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