CD34 (clone BIRMA K3) PE, anti-human

Decription:
Mouse monoclonal antibody to human CD34 PE conjugate. The CD34 antigen is a 105-120 kDa single transmembrane glycoprotein encoded by a gene located on chromosome 1. It is expressed on most haematopoietic colony forming cells from human bone marrow, including unipotent (BFU-E, CFU-GM, CFU-meg, CFU-Eo, CFU-Osteoclast) and multipotent progenitors (CFU- mix or CFU-GEMM, pre-CFU, CFU-Blast)2-4. CD34 appears to be expressed at the highest levels on the earliest progenitors, and to decrease progressively with maturation. CD34 is a stage-specific, rather than a lineage- specific, leucocyte differentiation antigen.

Synonyms: CD34, Hematopoietic progenitor cell antigen CD34 protein

Clone: BIRMA K3

Isotype: Mouse IgG1, kappa light chain

Immunogen: Immunogen was produced from a mouse hybridoma derived from the fusion of Balb/c spleen cells with X63Ag8.653 myeloma cells.

Reactivity: human

Form: PE-conjugate, in PBS buffer, pH 7.2 containing 0.1% sodium azide, 1% BSA

Applications: Flow Cytometry, Immunofluorescence

Working Dilution: 2ul per test (may change from lot to lot)

Storage: 2-8C, do not freeze

References:
1. Molgaard HV, Spurr NK, Greaves MF (1989). The hemopoietic skin cell antigen, CD34, is encoded by a gene located on chromosome
1. Leukemia 3:773-6.
2. Civin CI, Trischmann TM, Fackler MJ, Bernstein ID, Bohring HJ, Campos L, et al (1989). M7.
1. Report on the CD34 cluster workshop. In: Knapp W et al. eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford-New York-Tokyo: Oxford University Press. 818-25.
3. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwarts JF, Shaper JH (1984). Antigenic analysis of hematopoiesis. III. A hamatopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol 133:157-65.
4. Civin CI, Strauss LC, Fackler MJ, Trischmann TM, Wiley JM, Loken MR (1990). Positive stem cell selection - basic science. Prog Clin Biol Res 333:387- 402.
5. Loken MR, Shah VO, Hollander Z, Civin CI (1988). Flow cytometric analysis of normal B lymphoid development. Pathol Immunopathol Res 7:357-70.
6. Merle-Beral H, Cong Duc LN, Lebland V, Boucheix C, Michel A, Chastang C, et al (1989). Diagnostic and prognostic significance of myelomonocytic cell surface antigens in acute myeloid leukaemia. Br J Haematol 73:323-30.
7. Campos L, Guyotat D, Archimbaud E, Devaux Y, Treille D, Larese A, et al (1989). Surface marker expression in adult acute myeloid leukaemia: correlations with initial characteristics, morphology and response to therapy. Br J Haematol 72:161-6.
8. Batinic D, Tindle R, Boban D, Tiefenbach A, Rajic L, Labar B, et al (1989). Expression of haematopoietic progenitor cell-associated antigen BI-3C5/CD34 in leukaemia. Leuk Res 13:83-5.
9. Lai RB, Edison LJ, Chused TM (1988). Fixation and long-term storage of human lymphocytes for surface marker analysis by flow cytometry. Cytometry 9:213-9.
10. Haffkes HG et al, (1996) BIRMA-K3, a new monoclonal antibody for CD34 immunophenotyping and stem and progenitor cell assay. J Hematother. 5 (3):261-70.
1
1. Kishimoto T. et al, (eds) (1997) Leucocyte Typing VI: Sixth international workshop and conference on human leucocyte differentiation antigens, Japan, 1996 Garland Publishing Inc.
12. Arseniev L. et al, (1999) Comparative evaluation of commonly used clones and fluorochrome conjugates of monoclonal antibodies for CD34 antigen detection. J Hematother Stem Cell Res. 8 (5):547-59.
13. Macey MG et al, (1997) How should CD34+ cells be analysed? A study of three classes of antibody and five leucocyte preparation procedures. J Immunol Methods. 26; 204(2):175-88.
14. Serke S, Huhn D. (1996) Expression of class I, II and III epitopes of the CD34 antigen by normal and leukemic hemopoietic cells. Cytometry. 15; 26(2):154-60