CD354 (TREM1) polyclonal, anti-mouse

Quantity: 100 µg

Background:
Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.

Description:
Rabbit IgG polyclonal antibody for Triggering receptor expressed on myeloid cells 1 (TREM1) detection. Tested with WB, IHC-P, IHC-F in Mouse.

Synonyms:
CD354 antibody, OTTMUSP00000018206 antibody, TREM 1 antibody, TREM-1 antibody, TREM1 antibody, TREM1_HUMAN antibody, Triggering receptor expressed on monocytes 1 antibody, Triggering receptor expressed on myeloid cells 1 antibody, Triggering receptor TREM 1 antibody, Triggering receptor TREM1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle of mouse TREM1(74-90aa FTRPSEVHMGKFTLKHD).A synthetic peptide corresponding to a sequence in the middle of mouse TREM1(74-90aa FTRPSEVHMGKFTLKHD).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse

Applications: WB, IHC-P, IHC-F

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.