CD36 (clone 185-1G2), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
CD36 is a 80-90 kDa protein, expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV, and V, P.falciparum malaria-infected erythrocytes, and sickle erythrocytes. CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. 185-1G2 blocks adhesion of parasitized red blood cells to CD36 and strongly inhibits collagen-induced platelet aggregation (epitope within aa155-183; VIth HLDA workshop).

Species reactivity:
Positive: human.

Applications:
Demonstration of CD36. Staining of TSH activated endothelial cells, granular layer of skin, monocytes/macrophages, erythroblast, B-cell (weak), adipocytes.
Flow cytometry: +
Frozen sections: +
Immunohistology: +

Positive control: HEL, U937 or C32 melanoma cells. Platelets, monocytes, macrophages, umbilical vein endothelium, microvascular endothelial cells in tonsil

References:
- Kishimoto T. et al. Leucocyte typing VI, 636- 643 and 1136, Garland Publishing, Inc., New York and London, (1997).