CD363 (S1P1) polyclonal, anti-human, mouse, rat

Protein Family: Cellular Antigens, Receptors and Channels

Pathway and Disease: Cell Motility, Signal Transduction, Signaling Molecules and Interaction

Description:
Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P). One of its receptors S1P1 binds S1P with high specificity and affinity; agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling. At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.

Alternate Names: S1P1, Sphingosine 1-phosphate receptor 1, S1PR1, EDG1, ECGF1, CHEDG1, S1P receptor 1, Endothelial differentiation G-protein coupled receptor 1, CD363

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500

Accession No.: NP_001391

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human S1P1.

Format: Each vial contains 0.1 mg IgG (1 mg/ml) in PBS pH 7.4 with 0.02% sodium azide. Antibody was purified by immunogen affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.