CD3D polyclonal, anti-human

Background:
The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ΩΩ) or a heterodimer of zeta and eta chains (Ωh). The Ω and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the Ω chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the Ω chain ITAMs has been solved.

Alternative Name:
CD3D, CD3 antigen delta subunit, CD3 delta, CD3d, CD3d antigen delta polypeptide (TiT3 complex), CD3d molecule delta (CD3-TCR complex), CD3D, IMD19, OKT3 delta chain, T cell receptor T3 delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain, T3D,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: CD3D polyclonal antibody detects endogenous levels of CD3D protein.

Immunogen:
recombinant protein

MW: 19 kDa

Swiss-Prot: P04234(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.