CD3e (T-Cell Marker) polyclonal, anti-human, Predicted to react with ape, orangutan, gibbon, chimpanzee, monkey, elephant, horse, cow, sheep, pig, dog, cat, rabbit, rat, mouse, guinea pig, hamster, opossum, frog and chicken.

Description:
Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as γ, δ, ε, ζ, and η) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Synonyms:
CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Reactivity: human, Predicted to react with ape, orangutan, gibbon, chimpanzee, monkey, elephant, horse, cow, sheep, pig, dog, cat, rabbit, rat, mouse, guinea pig, hamster, opossum, frog and chicken.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (1-2ug/ml for 60 minutes at RT)
Optimal dilution for a specific application should be determined.

Positive Control:
Jurkat cells. Tonsil and lymph node

Molecular Weight:
20kDa

Concentration:
0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.