CD40 polyclonal, anti-human, mouse, rat

Background:
Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. A receptor ligand pair central to the trans-mission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif.

Alternative Name:
CD40, TNFRSF5, AI326936, B cell associated molecule CD40, B cell surface antigen CD40, B cell-associated molecule, B-cell surface antigen CD40, Bp50, CD 40, CD40, CD40 antigen (TNF receptor superfamily member 5), CD40 antigen, CD40 molecule, CD40 molecule, TNF receptor superfamily member 5, CD40 protein, CD40 type II isoform, CD40L receptor, CDw40, GP39, HIGM1, IGM, IMD3, MGC9013, Nerve growth factor receptor related B lymphocyte activation molecule, OTTHUMP00000031699, OTTHUMP00000031700, p50, T-BAM, TBAM, TNF receptor superfamily member 5, TNFRSF5, TNR5, TRAP, Tumor necrosis factor receptor superfamily , member 5, Tumor necrosis factor receptor superfamily member 5, Tumor necrosis factor receptor superfamily member 5 precursor, Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a,

Application Dilution:
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: CD40 polyclonal antibody detects endogenous levels of CD40 protein.

Immunogen:
Recombinant protein within human CD40 aa 10-250.

MW: Predicted band size 31kDa

Swiss-Prot: P25942(Human) P27512(Mouse)

Purification & Purity:
Protein affinity purified.

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.