CD41 (clone 5B12) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human CD4. Affinity purified. Integrin alpha 2b (CD41) is a 135 kDa calcium-dependent glycoprotein consisting of a heavy chain (GPIIb alpha; 120kDa) and a light chain (GPIIb beta; 23kDa) linked by a single disulphide bond. The alpha chain contains four calcium-binding sites and is entirely extracellular, while the beta chain has extracellular, transmembrane and cytoplasmic domains.

Synonyms: CD41, Integrin alpha 2b, 135 kDa calcium-dependent glycoprotein consisting of GPIIb alpha and GPIIb beta

Clone: 5B12

Isotype: Mouse IgG1, kappa light chain

Immunogen: 5B12 was made in response to immunisation with normal human platelets.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: MAIPA, Immunofluorescence

Working Dilution: 1:10 dilution for MAIPA, 1:20 Immunofluorescence

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Sun QH, Newman PJ. CD guide. CD41. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan . New York, London: Garland Publishing Inc.; 1997. p. 1139.
2. Vinci G, Tabilio A, Deschamps JF, van Haeke D, Henri A, Guichard J, et al. Immunological study of in vitro maturation of human megakaryocytes. Br J Haematol 1984; 56:589-605.
3. Honda S, Felding-Habermann B, Loftus J, Annis D, Kunicki TJ. CD41/CD61 cluster workshop report: localization of epitopes on integrins aIIb beta 3 (CD41/CD61) and av beta 3 (CD51/CD61). In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston , USA . Oxford , New York , Tokyo : Oxford University Press; 1995. p. 1293-8.
4. Kiefel, V., Santoso, S., Weisheit, M. & Mueller-Eckhardt, C. (1987) Monoclonal antibody-specific immobilization of platelet antigens (MAIPA): a new tool for the identification of platelet reactive antibodies. Blood, 70, 1722-1726.