CD44 (H-CAM) (clone 145-23), anti-human, mouse

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: SP2/0.



Specifications:
145-23 reacts with CD44, a type 1 transmembrane glycoprotein providing cell-cell and cell-matrix adhesion while linked to the cytoskeleton. It is involved in haematopoiesis, lymphocyte homing and activation, and tumor metastasis. It binds to fibrin, hyaluronate and other matrix proteins. On tumors CD44H is highly expressed, suggesting an important role in progression for this isoform. CD44 also forms the protein backbone of the human erythrocyte Lutheran antigen system. The epitope of 145-23 is resistant to (chemo)trypsin digestion.

Species reactivity:
Positive: human, mouse.

Applications:
CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: HeLa cells, paracortex in tonsil or lymph node, human breast cancer.

References:
- Leukocyte typing IV. White cell differentiation antigens. Proceedings of the 4th International Workshop and Conference, (Knapp, W et al., eds) Oxford University Press; (1989. p. 619-22).
- Nishina S. et al., Graefes Archiv. Clin. Exp. Opthalm. 235:92-96 (1997).
- Horny H.P. et al. Virchows Arch.429:91-94 (1996)